• Chorus2: design of genome‐scale oligonucleotide‐based probes for fluorescence in situ hybridization

    1 month ago - By Wiley

    Summary
    Oligonucleotide ‐fluorescence in situ hybridization has rapidly becoming the new generation of FISH technique in plant molecular cytogenetics research. Genome‐scale identification of single copy oligos is the foundation of successful oligo‐FISH experiments. Here, we introduce Chorus2, a software that is developed specifically for oligo selection. We demonstrate that Chorus2 is highly effective to remove all repetitive elements in selection of single copy oligos, which is critical for the development of successful FISH probes. Chorus2 is more effective than Chorus, the original...
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