• CRISPR/Cas9 induces exon skipping that facilitates development of fragrant rice

    6 days ago - By Wiley

    Splicing of precursor‐mRNAs is a critical biological process of gene expression. Normally, pre‐mRNAs are processed by spliceosomes to produce mature mRNAs by removing introns at 5′‐ and 3′‐ splice sites based on the canonical GU‐AG rule. Mutation at either the intron donor or acceptor sites should cause mRNA mis‐splicing. Recently, an exon skipping method has been developed to generate loss of gene function in mammalian cells using base editors to mutate nucleotides at the acceptor sites .
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