• NMF-RI: Blind spectral unmixing of highly mixed multispectral flow and image cytometry data

    8 days ago - By Oxford Journals

    AbstractMotivationRecent advances in multiplex immunostaining and multispectral cytometry have opened the door to simultaneously visualizing an unprecedented number of biomarkers both in liquid and solid samples. Properly unmixing fluorescent emissions is a challenging task, which normally requires the characterization of the individual fluorochromes from control samples. As the number of fluorochromes increases, the cost in time and use of reagents becomes prohibitively high. Here we present a fully-unsupervised blind spectral unmixing method for the separation of fluorescent emissions in...
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