• Blinking Statistics and Molecular Counting in direct Stochastic Reconstruction Microscopy

    1 month ago - By Oxford Journals

    AbstractMotivationMany recent advancements in single molecule localisation microscopy exploit the stochastic photo-switching of fluorophores to reveal complex cellular structures beyond the classical diffraction limit. However, this same stochasticity makes counting the number of molecules to high precision extremely challenging, preventing key insight into the cellular structures and processes under observation.ResultsModelling the photo-switching behaviour of a fluorophore as an unobserved continuous time Markov process transitioning between a single fluorescent and multiple dark states...
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